Caracteristicile genetice moleculare ale tulpinilor de Mycobacterium tuberculosis izolate de la pacienții cu tuberculoză nou identificați din Republica Azerbaidjan.
DOI:
https://doi.org/10.52692/1857-0011.2024.2-79.04Keywords:
Drug-resistant tuberculosis, „TB-TEST”, molecular genetic methodAbstract
In order to facilitate the early diagnosis of drug-resistant tuberculosis, a total of 198 sputum samples were subjected to analysis using the “TB-TEST” molecular genetic diagnostic kit. The samples were obtained from primary patients presenting with a range of clinical forms of pulmonary tuberculosis. The use of a molecular-genetic assay with high sensitivity and specificity for the determination of drug susceptibility in the tuberculosis pathogen markedly accelerates the diagnosis process, enabling the prescription of a rational therapeutic regimen within a timeframe of 2-3 days upon admission to the clinic. The method simultaneously identifies mutations in the rpoB , katG , inhA , ahpC , embB , rrs , eis , gyrA , gyrB genes, and determines the primary clinically significant genotypes of M. tuberculosis . This enables prompt and targeted treatment adjustments.
References
Global tuberculosis report 2024. Geneva: World Health Organization; 2024.
Farhat M., Cox H., Ghanem M., et al. Drug-resistant tuberculosis: a persistent global health concern. Nat. Rev. Microbiol., 2024; 22(10):617-35.
Hameed H.M.A., Islam M.M., Chhotaray C., et al. Molecular Targets Related Drug Resistance Mechanisms in MDR-, XDR-, and TDR-Mycobacterium tuberculosis Strains. Front. Cell. Infect. Microbiol., 2018; 8:114.
Swain, S.S., Sharma, D., Hussain, T., & Pati, S. Molecular mechanisms of underlying genetic factors and associated mutations for drug resistance in Mycobacterium tuberculosis. Emerg. Microbes & Infect., 2020; 9(1):1651–63.
Cole ST, Brosch R, Parkhill J, et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature, 1998; 393(6685):537-44.
Sachan R.S.K, Mistry V., Dholaria M., et al. Overcoming Mycobacterium tuberculosis Drug Resistance: Novel Medications and Repositioning Strategies. ACS Omega, 2023; 8(36):32244-57.
Walker T.M., Miotto P., Köser C.U., et al. The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: A genotypic analysis. Lancet Microbe, 2022; 3(4):e265-e273.
Zürcher K, Reichmuth ML, Ballif M, et al. Mortality from drug-resistant tuberculosis in high-burden countries comparing routine drug susceptibility testing with whole-genome sequencing: a multicentre cohort study. Lancet Microbe, 2021; 2(7): e320-e330.
Chen Y., Chen W., Cheng Z., et al. Global burden of HIV-negative multidrug- and extensively drug-resistant tuberculosis based on Global Burden of Disease Study 2021. Science in One Health., 2024; 3: 100072.
Jang J.G., Chung J.H. Diagnosis and treatment of multidrug-resistant tuberculosis. Yeungnam Univ. J. Med., 2020; 37(4): 277-85.
Sanchini A., Lanni A., Giannoni F., Mustazzolu A. Exploring diagnostic methods for drug-resistant tuberculosis: A comprehensive overview. Tuberculosis (Edinb)., 2024; 148: 102522.
Antimycobacterial Susceptibility Testing Group. Updating the approaches to define susceptibility and resistance to anti-tuberculosis agents: implications for diagnosis and treatment. Eur. Respir. J., 2022; 59(4): 2200166.
Nandlal L., Perumal R., Naidoo K. Rapid Molecular Assays for the Diagnosis of Drug-Resistant Tuberculosis. Infect. Drug Resist., 2022; 15: 4971-84.
Nguyen T.N.A, Anton-Le Berre V., Bañuls A.L., Nguyen T.V.A. Molecular Diagnosis of Drug- Resistant Tuberculosis; A Literature Review. Front. Microbiol., 2019; 10: 794.
Khan Z., Ualiyeva D., Jamal K., et al. Molecular diagnostics and potential therapeutic options for mycobacterium tuberculosis: Where we stand. Medicine in Omics, 2023; 8: 100022.
Беспятых Ю.А., Шитиков Е.А., Зименков Д.В., и др. Определение лекарственной устойчивости и генотипирование клинических штаммов Mycobacterium tuberculosis при помощи экспериментального набора «ТБ-ТЕСТ». Пульмонология, 2013; 4: 77-81.
Zimenkov D.V., Kulagina E.V., Antonova O.V., et al. Simultaneous drug resistance detection and genotyping of Mycobacterium tuberculosis using a low-density hydrogel microarray. J. Antimicrob. Chemother., 2016; 71(6): 1520-31.
Nosova E., Zimenkov D., Khakhalina A., et al. A comparison of the Sensititre MycoTB Plate, the Bactec MGIT 960, and a microarray-based molecular assay for the detection of drug resistance in clinical isolates of Mycobacterium tuberculosis in Moscow, Russia. PLoS One, 2016; 11(11): e0167093.
Gryadunov D.A., Shaskolskiy B.L., Nasedkina T.V., et al. The EIMB hydrogel microarrays technology: thirty years later. Acta Naturae, 2018; 4(39): 4-18.
Shea J, Halse TA, Kohlerschmidt D, et al. Low- Level Rifampin Resistance and rpoB Mutations in Mycobacterium tuberculosis: an Analysis of Whole- Genome Sequencing and Drug Susceptibility Test Data in New York. J. Clin. Microbiol., 2021; 59(4): e01885-20.
Downloads
Published
License
Copyright (c) 2025 Bulletin of the Academy of Sciences of Moldova. Medical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
